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Standards and QC samples were prepared by spiking pooled non-smokers urine with the analytes, spanning the expected concentration ranges

Because patches are commercially available only in fixed strengths and are usually used in step-down regimens, we could not adjust the dosing of this product

Finally, the nicotine concentrations of the aerosol droplets (the nicotine concentrations of the liquid phase collected by the DLPI cascade impactor) were calculated by dividing the total mass of nicotine (as determined by LCMS) by the total mass (or the volume, if the density was known) of the liquid aerosol droplets (as determined gravimetrically of the DLPI impaction plates)

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