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However, NASEM also concluded there is insufficient evidence from randomized controlled trials about the effectiveness of e-cigarettes as cessation aids compared with no treatment or to FDAapproved smoking cessation treatments

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For analysis of the total nitrogen (TN), total phosphorus (TP), total alkaloids (TA), amylum, protein, pH, petroleum ether extract (PEE), and total free amino acids (TFAA), the samples were pulverized in liquid nitrogen using a mortar and pestle and stored in an ultra-low-temperature freezer at 80C