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It also included questions on e-cigarette and cigarette use, including nicotine percentage, preferred flavors, reasons for use, symptoms after use, and promotion of e-cigarettes

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and FAPEMIG APQ-00823/16 and UFOP 23109.003268/2017-4 to F.S.B

Studies have highlighted the impact of nicotine on sperm DNA methylation, indicating variable methylation levels at specific genetic loci or CpG islands leading to potential adverse health outcomes in offspring, such as increased spontaneous locomotor activity and attention deficits by altering methylation levels in the promoter of Dat 20,21,22