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Combining topical GHK-CU with injectable GHK-CU without accounting for total copper load

NAD+/NADH ratio assays, SIRT1 activity assays, and mitochondrial respiration measurements (such as oxygen consumption rate via extracellular flux analysis) are standard methods for studying this combination's proposed effects in cell-based models, typically comparing single-compound and combined-compound treatment conditions against untreated controls

In Health and Happiness, Kelly Harrington, MS, RDN Registered Dietitian Nutritionist for Healthy Goods References: 1

Typically, low levels of ROS are associated with the maintenance of stem cell and self-renewal capacity, whereas elevated ROS levels can drive differentiation or induce cellular senescence