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doi: 10.1093/humupd/dmx016 159 ChenCZhouYHuCWangYYanZLiZet al

Samples were diluted in 6-salt PBS with or without GSH to twice the concentration required to obtain an OD of 1.0 in D antigen ELISA, duplicate samples heated for 10 min at each temperature were then diluted 1:1 with 2% dried milk in 6-salt PBS and cooled on ice

Need for maintenance dose: Need Of Maintenance Dose-Picture Courtesy: Health Cleveland Clinic Initially you administer dose every week for almost 6 weeks

From topical research: Most commonly reported observation: mild transient redness at application site (attributed to vascular activity from VEGF stimulation) No systemic adverse events reported in human topical studies at standard concentrations No evidence of carcinogenicity or mutagenicity in available in vitro models From injectable research (preclinical): At supratherapeutic doses in animal models, mild injection site reactions have been noted No organ toxicity observed in standard preclinical safety panels No immunogenicity concerns identified (consistent with its endogenous structure) Copper considerations: GHK-Cu delivers a small amount of bioavailable copper