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Furthermore, molecular hydrogen may help preserve NADPH levels by: Protecting glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme in the pentose phosphate pathway that generates NADPH Reducing overall cellular oxidative status, decreasing NADPH consumption by other systems Preserving mitochondrial function, supporting energy metabolism that indirectly maintains NADPH production This conservation of NADPH availability ensures that glutathione reductase has adequate cofactor supply to perform its glutathione recycling function, especially during periods of increased oxidative challenge