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The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis

For adults over 65, no dose adjustment is required, but careful monitoring for adverse effects is prudent as older adults often have multiple comorbidities and medications that increase complication risk

The mass stays fixed at 10 mg

This ensures your approach is safe and effective, being made to individual health needs while minimizing unnecessary risks